From Wikipedia, the free encyclopedia
Protein-coding gene in the species Homo sapiens
Ribonuclease H1 also known as RNase H1 is an
enzyme that in humans is encoded by the RNASEH1
gene .
[5]
[6]
[7] The RNase H1 is a non-specific
endonuclease and catalyzes the cleavage of
RNA via a hydrolytic mechanism.
References
^
a
b
c
GRCh38: Ensembl release 89: ENSG00000171865 –
Ensembl , May 2017
^
a
b
c
GRCm38: Ensembl release 89: ENSMUSG00000020630 –
Ensembl , May 2017
^
"Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .
^
"Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .
^
"Entrez Gene: ribonuclease H1" .
^ ten Asbroek AL, van Groenigen M, Jakobs ME, Koevoets C, Janssen B, Baas F (June 2002). "Ribonuclease H1 maps to chromosome 2 and has at least three pseudogene loci in the human genome". Genomics . 79 (6): 818–23.
doi :
10.1006/geno.2002.6776 .
PMID
12036296 .
^ Nowotny M, Gaidamakov SA, Ghirlando R, Cerritelli SM, Crouch RJ, Yang W (October 2007).
"Structure of human RNase H1 complexed with an RNA/DNA hybrid: insight into HIV reverse transcription" . Mol. Cell . 28 (2): 264–76.
doi :
10.1016/j.molcel.2007.08.015 .
PMID
17964265 .
Further reading
Nowotny M, Cerritelli SM, Ghirlando R, et al. (2008).
"Specific recognition of RNA/DNA hybrid and enhancement of human RNase H1 activity by HBD" . EMBO J . 27 (7): 1172–81.
doi :
10.1038/emboj.2008.44 .
PMC
2323259 .
PMID
18337749 .
Lima WF, Wu H, Nichols JG, et al. (2003).
"Human RNase H1 uses one tryptophan and two lysines to position the enzyme at the 3'-DNA/5'-RNA terminus of the heteroduplex substrate" . J. Biol. Chem . 278 (50): 49860–7.
doi :
10.1074/jbc.M306543200 .
PMID
14506260 .
Lima WF, Rose JB, Nichols JG, et al. (2007). "The positional influence of the helical geometry of the heteroduplex substrate on human RNase H1 catalysis". Mol. Pharmacol . 71 (1): 73–82.
doi :
10.1124/mol.106.025429 .
PMID
17028157 .
S2CID
27765676 .
Lima WF, Nichols JG, Wu H, et al. (2004).
"Structural requirements at the catalytic site of the heteroduplex substrate for human RNase H1 catalysis" . J. Biol. Chem . 279 (35): 36317–26.
doi :
10.1074/jbc.M405035200 .
PMID
15205459 .
Otsuki T, Ota T, Nishikawa T, et al. (2005).
"Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries" . DNA Res . 12 (2): 117–26.
doi :
10.1093/dnares/12.2.117 .
PMID
16303743 .
Wu H, Lima WF, Crooke ST (1999).
"Properties of cloned and expressed human RNase H1" . J. Biol. Chem . 274 (40): 28270–8.
doi :
10.1074/jbc.274.40.28270 .
PMID
10497183 .
Frank P, Braunshofer-Reiter C, Pöltl A, Holzmann K (1998). "Cloning, subcellular localization and functional expression of human RNase HII". Biol. Chem . 379 (12): 1407–12.
doi :
10.1515/bchm.1998.379.12.1407 .
PMID
9894807 .
S2CID
19692186 .
Wu H, Lima WF, Zhang H, et al. (2004).
"Determination of the role of the human RNase H1 in the pharmacology of DNA-like antisense drugs" . J. Biol. Chem . 279 (17): 17181–9.
doi :
10.1074/jbc.M311683200 .
PMID
14960586 .
Hillier LW, Graves TA, Fulton RS, et al. (2005).
"Generation and annotation of the DNA sequences of human chromosomes 2 and 4" . Nature . 434 (7034): 724–31.
Bibcode :
2005Natur.434..724H .
doi :
10.1038/nature03466 .
PMID
15815621 .
Lima WF, Wu H, Nichols JG, et al. (2003).
"Human RNase H1 activity is regulated by a unique redox switch formed between adjacent cysteines" . J. Biol. Chem . 278 (17): 14906–12.
doi :
10.1074/jbc.M211279200 .
PMID
12473655 .
ten Asbroek AL, van Groenigen M, Nooij M, Baas F (2002).
"The involvement of human ribonucleases H1 and H2 in the variation of response of cells to antisense phosphorothioate oligonucleotides" . Eur. J. Biochem . 269 (2): 583–92.
doi :
10.1046/j.0014-2956.2001.02686.x .
PMID
11856317 .
Gerhard DS, Wagner L, Feingold EA, et al. (2004).
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)" . Genome Res . 14 (10B): 2121–7.
doi :
10.1101/gr.2596504 .
PMC
528928 .
PMID
15489334 .
Ota T, Suzuki Y, Nishikawa T, et al. (2004).
"Complete sequencing and characterization of 21,243 full-length human cDNAs" . Nat. Genet . 36 (1): 40–5.
doi :
10.1038/ng1285 .
PMID
14702039 .
Cerritelli SM, Crouch RJ (1998).
"Cloning, expression, and mapping of ribonucleases H of human and mouse related to bacterial RNase HI" . Genomics . 53 (3): 300–7.
doi :
10.1006/geno.1998.5497 .
PMID
9799596 .
Strausberg RL, Feingold EA, Grouse LH, et al. (2002).
"Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences" . Proc. Natl. Acad. Sci. U.S.A . 99 (26): 16899–903.
Bibcode :
2002PNAS...9916899M .
doi :
10.1073/pnas.242603899 .
PMC
139241 .
PMID
12477932 .
Cerritelli SM, Frolova EG, Feng C, et al. (2003).
"Failure to produce mitochondrial DNA results in embryonic lethality in Rnaseh1 null mice" . Mol. Cell . 11 (3): 807–15.
doi :
10.1016/S1097-2765(03)00088-1 .
PMID
12667461 .
Lima WF, Rose JB, Nichols JG, et al. (2007). "Human RNase H1 discriminates between subtle variations in the structure of the heteroduplex substrate". Mol. Pharmacol . 71 (1): 83–91.
doi :
10.1124/mol.106.025015 .
PMID
17028158 .
S2CID
24995048 .
External links
Overview of all the structural information available in the
PDB for
UniProt :
O60930 (Human Ribonuclease H1 (RNASEH1)) at the
PDBe-KB .