From Wikipedia, the free encyclopedia
Bismuth sulfite agar is a type of
agar media used to isolate
Salmonella species. It uses
glucose as a primary source of
carbon. Bismuth and
brilliant green (dye) both inhibit
gram-positive growth.
Bismuth sulfite agar tests the ability to use
ferrous sulfate and convert it to
hydrogen sulfide.
Bismuth sulfite agar typically contains (
w/v):
[1]
- 1.6%
bismuth sulfite Bi2(SO3)3
- 1.0% pancreatic
digest of
casein
- 1.0% pancreatic digest of animal
tissue
- 1.0%
beef extract
- 1.0%
glucose
- 0.8% dibasic
sodium phosphate
- 0.06%
ferrous sulfate • 7 water
- pH adjusted to 7.7 at 25 °C
This medium is boiled for sterility, not
autoclaved.
References