| County | Location | mi | km | Exit | Destinations | Notes |
|---|---|---|---|---|---|---|
| Leon | Tallahassee | 0.00 | 0.00 |  
US 319 /
SR 263 –
Crawfordville,
Tallahassee | Southern terminus of I-1 | |
| 0.00 | 0.00 | 1 | Orange Avenue | |||
| 0.00 | 0.00 | 2 |  
US 27 /
SR 20 –
Tallahassee,
Waukeenah | Southbound exit; northbound entrance | ||
| 0.00 | 0.00 | 3 |  
US 90 /
SR 10 to 
I-30 –
Tallahassee,
Jacksonville | |||
| 0.00 | 0.00 | 4 | 
SR 61 –
Tallahassee,
Bradfordville | |||
| 0.00 | 0.00 | 5A-B |
I-30 –
Pensacola,
Jacksonville | Four-level stack interchange | ||