Live attenuated influenza vaccine (LAIV) is a type of
influenza vaccine in the form of a
nasal spray that is recommended for the prevention of influenza. It was developed by the Syrian-American epidemiologist
Hunein Maassab.[5]
It is an attenuated live vaccine, unlike other influenza vaccines, which are
inactivated vaccines. Live attenuated influenza vaccine is administered
intranasally,[6] while inactivated vaccines are administered by
intramuscular injection. Live attenuated influenza vaccine is sold under the brand name FluMist Quadrivalent in the United States and the brand name Fluenz Tetra in the European Union.[7][4] FluMist was first introduced in 2003 by
MedImmune.[8][9][10]
Medical uses
The live attenuated influenza vaccine is used to provide protection against the flu caused by infection with influenza viruses.[11][12][13]
Contraindications
The use of the live attenuated influenza vaccine is
contraindicated, and should therefore not be used, in the following populations:
children <24 months of age, due to increased risk of wheezing[14]
individuals with a history of hypersensitivity to previous influenza vaccination.[14]
individuals with a history of hypersensitivity, especially
anaphylactic reactions, to eggs, egg proteins, gentamicin, gelatin, or arginine or to any other ingredient in the formulation [14]
People with a medical condition that places them at high risk for complications from influenza, including those with chronic heart or lung disease, such as asthma or reactive airways disease[15]
People with medical conditions such as diabetes or kidney failure or people with illnesses that weaken the immune system, or who take medications that can weaken the immune system[15]
Children less than 5 years old with a history of recurrent wheezing[15]
People who have a severe allergy to chicken eggs or who are allergic to any of the nasal spray vaccine components[15]
Production
The live attenuated vaccine is based on a flu strain that does not cause disease, that replicates well at relatively cold temperatures (about 25 °C, for incubation purposes), and replicates poorly at body temperature (which minimizes risk to humans). Genes that code for surface proteins (targeted
antigens) are combined with this host using genetic
reassortment from strains that are projected to be circulating widely in the coming months. The resulting viruses are then incubated in chicken eggs and chick kidney cells. To make the refrigerated version, the virus is purified in
centrifuges through a
sucrose gradient, then packaged with sucrose,
phosphate,
glutamate,
arginine, and
gelatin made from pigs that has been
hydrolyzed with acid.[16]
Risks
Even though the virus in the live attenuated influenza vaccine is attenuated (low in
virulence), it is still a living virus, and may cause an
infection with complications in people with weakened immune systems or other underlying medical conditions. The live attenuated influenza vaccine is recommended only for people 2–49 years of age, and people who have a weakened immune system, pregnant women, and people with certain chronic diseases may not be eligible to receive live attenuated influenza vaccine.[17] In contrast, inactivated virus vaccines contain no living virus, and cannot cause a live infection. Persons receiving the live attenuated influenza vaccine may shed small amounts of the vaccine virus during the first week. People coming in contact with the vaccinated person are not considered to be at risk, unless their immune systems are severely weakened (for example, bone marrow transplant recipients) and possible recombination with other (wild or live vaccine) flu strains.[5]
The FDA initially approved the live attenuated influenza vaccine only for healthy people aged 5 to 49 because of concerns over possible side effects. The live attenuated influenza vaccine is approved and recommended for healthy children 24 months of age and older. The FDA approved the unfrozen refrigerated version for the same age group (ages 5–49) in August 2006, following completion of phase III clinical trials.[19]
The cold-adapted influenza vaccine version of the vaccine is called CAIV-T, and is stable for storage in a refrigerator, rather than requiring freezer storage as did the originally-approved formulation. Approved for the 2007-2008 flu season,[10] the refrigerated formulation can be distributed more economically, so that the price differential with shots (which had hampered sales of the original frozen version of FluMist) is now largely eliminated. FluMist was initially priced higher than the injectable vaccines, but sold only 500,000 of the four million doses it produced its first year on the market, despite a comparative shortage of flu vaccine in fall 2004.[20] The price was sharply lowered the next year, and the company reported distributing 1.6 million doses in 2005.[21] Because of the price drop, despite selling almost three times as many doses in 2005, the company reported $21 million in FluMist sales, compared to $48 million the previous year.[22]
Society and culture
MedImmune is one company that manufactures the live attenuated influenza vaccine, which it sells under the brand name FluMist in the United States and the brand name Fluenz Tetra[4] in the European Union. For the 2010–2011 flu season, FluMist was the only live attenuated influenza vaccine approved by the FDA for use in the US.[23][24] All other FDA-approved lots were inactivated virus vaccines.[citation needed] In September 2009, a live attenuated influenza vaccine for the novel H1N1 influenza virus was approved[25] and the seasonal intranasal vaccine was approved by the
European Medicines Agency (EMA) for use in the European Union in 2011.[3] The quadrivalent version was approved for use in the European Union in 2013.[4]
As of 2007[update], the only other company holding live attenuated influenza vaccine rights is BioDiem of Australia.[26] BioDiem licensed rights to private production of the vaccine in China to Changchun BCHT Biotechnology, which also holds public rights for production in China sublicensed from the
World Health Organization.[27]
It was the first and, as of 2007[update], the only live attenuated vaccine for influenza available outside of Europe.[28] In September 2009, a live attenuated influenza vaccine for the novel H1N1 influenza virus was approved.[25] In 2011, the vaccine was approved by the
European Medicines Agency (EMA) for use in the European Union under the brand name Fluenz.[3][29]
The live attenuated influenza vaccine is designed to be quickly modifiable to present the surface antigens of seasonal flu. The modifiability could also allow it to be quickly customized as a vaccine against a pandemic influenza if one were to emerge. In light of the
global spread of H5N1, ways of reducing
human mortality in the event of an H5N1 pandemic have been investigated. Modifying FluMist to serve as a specific human H5N1 vaccine is among the measures studied.[32]
In September 2006, the
National Institute of Allergy and Infectious Diseases (NIAID) reported that inoculation with a live attenuated influenza vaccine modified to present the surface antigens of certain H5N1 variants provided broad protection against other H5N1 variants in the mouse and ferret models.[34] Attenuated live viruses were found protective against H5N1 in mice and chickens in a 2009 study.[35]
"Several trials have reported that live attenuated influenza vaccines can boost virus-specific CTLs as well as mucosal and serum antibodies and provide broad cross-protection against heterologous human influenza A viruses." (58, 59)[36] "[V]accine formulas inducing
heterosubtypic T cell–mediated immunity may confer broad protection against avian and human influenza A viruses."[36]