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DIDS
Names
Preferred IUPAC name
2,2′-(Ethene-1,2-diyl)bis(5-isothiocyanatobenzene-1-sulfonic acid)
Other names
5-Isothiocyanato-2-[2-(4-isothiocyanato-2-sulfophenyl)ethenyl]benzenesulfonic acid
Identifiers
Abbreviations
DIDS
6543839
ChEBI
ChemSpider
ECHA InfoCard
100.152.489
KEGG
MeSH
4,4'-Diisothiocyanostilbene-2,2'-disulfonic+acid
UNII
InChI=1S/C16H10N2O6S4/c19-27(20,21)15-7-13(17-9-25)5-3-11(15)1-2-12-4-6-14(18-10-26)8-16(12)28(22,23)24/h1-8H,(H,19,20,21)(H,22,23,24)
Key: YSCNMFDFYJUPEF-UHFFFAOYSA-N
OS(=O)(=O)C1=C(C=CC2=C(C=C(C=C2)N=C=S)S(O)(=O)=O)C=CC(=C1)N=C=S
Properties
C 16 H 10 N 2 O 6 S 4
Molar mass
454.50 g·mol−1
Melting point
400 °C (752 °F; 673 K)
log P
4.72
Acidity (pK a )
-3.21, -1.428, -0.37, 0.23
Basicity (pK b )
13.77, 14.37, 15.425, 17.21
Except where otherwise noted, data are given for materials in their
standard state (at 25 °C [77 °F], 100 kPa).
Chemical compound
4,4′-Diisothiocyano-2,2′-stilbenedisulfonic acid (DIDS ) is an
anion exchange
inhibitor ,
[1] blocking reversibly, and later irreversibly,
exchangers such as
chloride-bicarbonate exchanger .
[2]
References
^ Jessen, Flemming; Sjøholm, C; Hoffmann, EK (1986), "Identification of the anion exchange protein of ehrlich cells: A kinetic analysis of the inhibitory effects of 4,4′-diisothiocyano-2,2′-stilbene-disulfonic acid (DIDS) and labeling of membrane proteins with3H-DIDS",
Journal of Membrane Biology , 92 (3): 195–205,
doi :
10.1007/BF01869388 ,
PMID
3783658 ,
S2CID
19244281
^ Lane, Michelle; Baltz, Jay M.; Bavister, Barry D. (1999), "Bicarbonate/Chloride Exchange Regulates
Intracellular pH of Embryos but Not Oocytes of the Hamster",
Biology of Reproduction , 61 (2): 452–457,
doi :
10.1095/biolreprod61.2.452 ,
PMID
10411526
Transporter
EAATs Tooltip Excitatory amino acid transporters
vGluTs Tooltip Vesicular glutamate transporters
Enzyme
GAH Tooltip Glutamine aminohydrolase (glutaminase)
AST Tooltip Aspartate aminotransferase
ALT Tooltip Alanine aminotransferase
GDH Tooltip Glutamate dehydrogenase
GS Tooltip Glutamine synthetase
GAD Tooltip Glutamate decarboxylase