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Helicobacter pylori colonized on the surface of regenerative epithelium, stained with the Warthin–Starry method.
Clusters of bacteria (arrow) shown on Warthin–Starry stain.

The Warthin–Starry stain (WS) is a silver nitrate-based staining method (a silver stain) used in histology. It was first introduced in 1920 by American pathologists Aldred Scott Warthin (1866–1931) and Allen Chronister Starry (1890–1973), for the detection of spirochetes. [1] [2] It has been considered a standard stain for the detection of spirochetes, [3] and is also used to stain Helicobacter pylori, Lawsonia intracellularis, [4] Microsporidia, [5] [6] and particulates. [7] It is also important for confirmation of Bartonella henselae, a causative organism in cat-scratch disease.

Warthin–Starry stains organisms dark brown to black, and the background light golden brown/golden yellow. [5]

See also

References

  1. ^ "Warthin-Starry stain". Merriam-Webster's Medical Dictionary. Retrieved 2009-09-04.
  2. ^ Warthin, AS; Starry, AC (1920). "A more rapid and improved method of demonstrating spirochetes in tissues (Warthin and Starry's cover-glass method)". American Journal of Syphilis. 4: 97–103.
  3. ^ "Special Stains in Histology". The Internet Pathology Laboratory. University of Utah Spencer S. Eccles Health Sciences Library. n.d. Retrieved 2009-09-04.
  4. ^ Huerta, B.; Arenas, A.; Carrasco, L.; et al. (Aug 2003). "Comparison of diagnostic techniques for porcine proliferative enteropathy (Lawsonia intracellularis infection)". Journal of Comparative Pathology. 129 (2–3): 179–185. doi: 10.1016/S0021-9975(03)00030-6. ISSN  0021-9975. PMID  12921724.
  5. ^ a b Field, A. S.; Marriott, D. J.; Hing, M. C. (1993). "The Warthin-Starry stain in the diagnosis of small intestinal microsporidiosis in HIV-infected patients" (PDF). Folia parasitologica. 40 (4): 261–6. ISSN  0015-5683. PMID  7516907.
  6. ^ Słodkowicz-Kowalska, A (2004). "Laboratory diagnostics of human microsporidiosis" (Free full text). Wiadomosci parazytologiczne. 50 (4): 679–89. ISSN  0043-5163. PMID  16862802.
  7. ^ Liu, H. (2008). "Warthin-starry Silver Method Showing Particulate Matter in Macrophage1". Biomedical and Environmental Sciences. 21: 85–9. doi: 10.1016/S0895-3988(08)60011-2. PMID  18478983.

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