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Additional information

Phototropins also regulate the movement of chloroplasts within the cell, notably chloroplast avoidance. It was thought that this avoidance serves a protective function to avoid damage from intense light [1], however an alternate study argues that the avoidance response is primarily to increase light penetration into deeper mesophyll layers in high light conditions [2].

Enzyme Activity

The crystal structure of the LOV2 domain of Phototropin-2 of Arabidopsis thaliana, generated using ChimeraX. Part of the LOV2 domain is hidden for clarity of the active site containing FMN. The dotted blue lines represent hydrogen bonds predicted as important in binding. In green are Cys426 and Arg427 residues which are crucial in photoactivity and FMN binding, respectively, with mutations resulting in total loss of function of the protein. [3] Upon photoexcitation, the sulfur (yellow) of Cys426 forms a covalent bond with the carbon 4 of FMN. ( PDBe: 4EEP)

Phototropins have two distinct light, oxygen, or voltage regulated domains (LOV1, LOV2) that each bind flavin mononucleotide (FMN). [3] The flavin mononucleotide is noncovalently bound to a LOV domain in the dark, but becomes covalently linked upon exposure to suitable light. [3] The formation of the bond is reversible once light is no longer present. [3] The forward reaction with light is not dependent on temperature, though low temperatures give increased stability of the covalent linkage, leading to a slower reversal reaction. [3]

Light excitation will lead to a conformational change within the protein, which allows for kinase activity. [4] There is also evidence to suggest that phototropins undergo autophosphorylation at various sites across the enzyme. [3] Phototropins trigger signaling responses within the cell, but it is unknown which proteins are phosphorylated by phototropins, or exactly how the autophosphorylation events play a role in signaling. [3]

Phototropins are typically found on the plasma membrane, but some phototropins have been found in substantial quantities on chloroplast membranes. [5] One study found that phototropins on the plasma membrane play a role in phototropism, leaf flattening, stomatal opening, and chloroplast movements, while phototropins on the chloroplasts only partially affected stomatal opening and chloroplast movement, [6] suggesting that the location of the protein in the cell may also play a role in its signaling function.


References

  1. https://academic.oup.com/jxb/article/73/18/6034/6628641?login=false#377404162 (main article)
  2. https://www-nature-com.aurarialibrary.idm.oclc.org/articles/nature01213 (chloroplasat avoidance is due to damaging blue light)
  3. https://academic.oup.com/plphys/article/183/3/1213/6116435 (contradictory to source 2, says avoidance is so light can penetrate into deeper mesophyl layers
  4. https://pubs.acs.org/doi/full/10.1021/bi9009192?casa_token=ENm0ptxp_e0AAAAA%3AoUHtFXwZPngoPC-QGR2CYYsQUGIKvrm-pp2kZs63G4jCAETZfpTMRdu6jkkmuURTz8GgcUfXkBOZNBCa (structural changes caused by light reaction)
  5. https://academic.oup.com/pcp/article/54/1/80/1903350 (phototropins being found localized on chloroplasts)
  6. https://academic.oup.com/plphys/article/183/1/304/6116383?login=false#251625468 (phototropins induce a different response based on location; plasma membrane vs chloroplast)
  7. https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2015.00637/full (phylogeny and evolution of phototropins, found across many species)
  1. ^ Kasahara, M., Kagawa, T., Olkawa, K., Suetsugu, N., Miyao, M., & Wada, M. (2002). Chloroplast avoidance movement reduces photodamage in plants. Nature, 420(6917). https://doi.org/10.1038/nature01213
  2. ^ Wilson, S., & Ruban, A. v. (2020). Rethinking the influence of chloroplast movements on non-photochemical quenching and photoprotection. Plant Physiology, 183(3). https://doi.org/10.1104/pp.20.00549
  3. ^ a b c d e f g Łabuz, J., Sztatelman, O., & Hermanowicz, P. (2022). Molecular insights into the phototropin control of chloroplast movements. In Journal of Experimental Botany (Vol. 73, Issue 18). https://doi.org/10.1093/jxb/erac271
  4. ^ Koyama, T., Iwata, T., Yamamoto, A., Sato, Y., Matsuoka, D., Tokutomi, S., & Kandori, H. (2009). Different role of the Jα helix in the light-induced activation of the LOV2 domains in various phototropins. Biochemistry, 48(32). https://doi.org/10.1021/bi9009192
  5. ^ Kong, S. G., Suetsugu, N., Kikuchi, S., Nakai, M., Nagatani, A., & Wada, M. (2013). Both phototropin 1 and 2 localize on the chloroplast outer membrane with distinct localization activity. Plant and Cell Physiology, 54(1). https://doi.org/10.1093/pcp/pcs151
  6. ^ Ishishita, K., Higa, T., Tanaka, H., Inoue, S. I., Chung, A., Ushijima, T., Matsushita, T., Kinoshita, T., Nakai, M., Wada, M., Suetsugu, N., & Gotoh, E. (2020). Phototropin2 contributes to the chloroplast avoidance response at the chloroplast-plasma membrane InterfAce1[CC-by]. Plant Physiology, 183(5). https://doi.org/10.1104/pp.20.00059
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