SR 341 begins at an
intersection with
US 11E in
Talbott (within the city limits of
Morristown, Tennessee) in
Hamblen County. It then enters
Jefferson County and heads south as Talbott–Kansas Road to an intersection where Talbott–Kansas Road travels south and SR 341 turns east onto North White Pine Road and continues as White Pine Road until its intersection with
SR 66. It enters
White Pine just before the SR 66 intersection, where it becomes Roy Messer Highway and then has an
interchange with
I-81 and continues east to meet its eastern terminus, an intersection with
US 25E.