This is the
talk page for discussing improvements to the
Environmental DNA article. This is not a forum for general discussion of the article's subject. |
Article policies
|
Find sources: Google ( books · news · scholar · free images · WP refs) · FENS · JSTOR · TWL |
![]() | Environmental DNA was nominated as a Natural sciences good article, but it did not meet the good article criteria at the time (January 6, 2022). There are suggestions on the review page for improving the article. If you can improve it, please do; it may then be renominated. |
![]() | This article is rated B-class on Wikipedia's
content assessment scale. It is of interest to the following WikiProjects: | |||||||||||||||||||||||||||||
|
![]() | This article has been
mentioned by a media organization:
|
![]() |
Daily pageviews of this article
A graph should have been displayed here but
graphs are temporarily disabled. Until they are enabled again, visit the interactive graph at
pageviews.wmcloud.org |
Hello all-- Here are the following papers I'll be using as my key references. Comments? Advice?
Leahc 10 ( talk) 04:21, 3 February 2017 (UTC)
Leahc 10 ( talk) 19:22, 25 January 2017 (UTC)
I am going to be editing this article over the next 2 months for my Advanced Genetics class at Western University. Any suggestions would be greatly appreciated! Asincl29 ( talk) 16:26, 16 October 2015 (UTC)
My proposed changes to this segment of the Wikipedia page reflect the changing nature of current environmental DNA research. The majority of eDNA work is being conducted with water collected from the water column, and the information available in the associated section of the Wikipedia page should reflect changes to this section of eDNA research and the more advanced methodology concerning that work.
See below for the proposed changes. In brackets are material already published on the page.
<The use of eDNA in aquatic sediment has been useful, but can even be applied to open water for present day study. Before eDNA, the main ways to study open water diversity was to use fishing and trapping, which requires funding and skilled labor, and other various resources, but eDNA only needs samples of water.> The majority of recent environmental DNA studies focus on samples taken from the water column [1][2]. Recent studies have included tests with fishes, amphibians, aquatic mammals, and other organisms [2]. More data is needed to determine the ability of environmental DNA samples to estimate population sizes [3]. Currently, many studies use presence/absence data as the response variable from environmental DNA tests. A volume of water (averaging ~2 L per sample) is filtered through a filter paper by use of a hand pump or peristaltic pump, and samples are stored for later laboratory analysis [4][5]. Samples filtered from water samples can be stored by freezing the samples or desiccating them in ethanol [6]. <This method is effective as pH of the water does not affect the DNA as much as previously thought, and can be made more sensitive with relative ease. Sensitivity is how likely the DNA marker will be present in the sampled water, and can be increased simply by taking more samples, having bigger samples, and increasing PCR. eDNA degrades relatively fast in the water column, which is very beneficial in short term conservation studies such as identifying what species are present.> [1] Jerde, C.L., Mahon, A.R., Chadderton, W.L. and Lodge, D.M., 2011. “Sight‐unseen” detection of rare aquatic species using environmental DNA. Conservation Letters, 4(2), pp.150-157. [2] Thomsen, P.F. and Willerslev, E., 2015. Environmental DNA–An emerging tool in conservation for monitoring past and present biodiversity. Biological Conservation, 183, pp.4-18. [3] Evans, N.T., Olds, B.P., Renshaw, M.A., Turner, C.R., Li, Y., Jerde, C.L., Mahon, A.R., Pfrender, M.E., Lamberti, G.A. and Lodge, D.M., 2016. Quantification of mesocosm fish and amphibian species diversity via environmental DNA metabarcoding. Molecular Ecology Resources, 16(1), pp.29-41. [4] Rees, H.C., Maddison, B.C., Middleditch, D.J., Patmore, J.R. and Gough, K.C., 2014. The detection of aquatic animal species using environmental DNA–a review of eDNA as a survey tool in ecology. Journal of Applied Ecology, 51(5), pp.1450-1459. [5] Pilliod, D.S., Goldberg, C.S., Arkle, R.S. and Waits, L.P., 2013. Estimating occupancy and abundance of stream amphibians using environmental DNA from filtered water samples. Canadian Journal of Fisheries and Aquatic Sciences, 70(8), pp.1123-1130. [6] Renshaw, M.A., Olds, B.P., Jerde, C.L., McVeigh, M.M. and Lodge, D.M., 2015. The room temperature preservation of filtered environmental DNA samples and assimilation into a phenol–chloroform–isoamyl alcohol DNA extraction. Molecular ecology resources, 15(1), pp.168-176. — Preceding unsigned comment added by AlexWiebe1 ( talk • contribs) 13:48, 9 November 2017 (UTC)
GA toolbox |
---|
Reviewing |
Reviewer: Jens Lallensack ( talk · contribs) 19:45, 2 January 2022 (UTC)
Few comments for a start, more later:
@ Jens Lallensack: thank you for those thoughtful comments. I'll address these issues over the next few days. — Epipelagic ( talk) 07:10, 3 January 2022 (UTC)
Closing note: Failed GA per request by the author. -- Jens Lallensack ( talk) 11:06, 6 January 2022 (UTC)
I was fixing some ref errors in this article; at the same time also cleaned up a bunch of duplicated refs mostly from the lead and cleaned up the selfDNA section, which goes a little way to addressing some of the ref based comments in the GA review above. Amkilpatrick ( talk) 14:26, 10 January 2022 (UTC)
The article currently contains only the positive aspects of eDNA. Increasingly, aspects concerning privacy and ethical concerns have been raised.
I'm not an expert in the field but perhaps someone more knowledgeable might add a section (or two sections?) covering such issues. Following are a few sources that discuss such issues:
- https://www.nytimes.com/2023/05/15/science/environmental-dna-ethics-privacy.html
- https://phys.org/news/2023-05-dna-samples-sand-air-ethical.html
- https://www.vice.com/en/article/88xq4v/human-dna-is-all-over-the-place-raising-privacy-concerns
- https://cosmosmagazine.com/people/ethics/edna-ethical-risks/
- https://www.bbc.co.uk/programmes/m001m4w7 31.51.14.148 ( talk) 20:55, 25 May 2023 (UTC)
This article is mainly about modern environmental DNA. I think the section on SedaDNA (sedimentary ancient DNA) is primarily of interest to people interested in Ancient DNA. I suggest moving the section to that article with a hatnote on this article {{about|modern environmental DNA|ancient sedimentary DNA (SedaDNA)|Ancient DNA}}. Dudley Miles ( talk) 19:13, 2 June 2023 (UTC)
Greetings,
In addition to the topics suggested for change by the reviewer, how about information on the history of the technique? I am lost trying to find any here.
I came here for the history of eDNA and was disappointed.
Thank you for your time,
Wordreader (
talk) 16:03, 7 April 2024 (UTC)