Serum amyloid A (SAA) proteins are a family of
apolipoproteins associated with
high-density lipoprotein (HDL) in plasma. Different isoforms of SAA are expressed constitutively (constitutive SAAs) at different levels or in response to inflammatory stimuli (
acute phase SAAs). These proteins are produced predominantly by the liver.[1]
Acute-phase serum amyloid A proteins
Acute-phase serum amyloid A proteins (A-SAAs) are secreted during the acute phase of
inflammation. These proteins have several roles, including the transport of
cholesterol to the
liver for secretion into the
bile, the recruitment of immune cells to inflammatory sites, and the induction of
enzymes that degrade
extracellular matrix. A-SAAs are implicated in several chronic inflammatory diseases, such as
amyloidosis,
atherosclerosis, and
rheumatoid arthritis.[2] Three acute-phase SAA
isoforms have been reported in mice, called
SAA1,
SAA2, and SAA3. During
inflammation, SAA1 and SAA2 are expressed and induced principally in the
liver, whereas SAA3 is induced in many distinct tissues. SAA1 and SAA2 genes are regulated in liver cells by the proinflammatory
cytokinesIL-1,
IL-6, and
TNF-α. Both SAA1 and SAA2 are induced up to a 1000-fold in mice under acute inflammatory conditions following exposure to bacterial
lipopolysaccharide (LPS).[2] Three A-SAA genes have also been identified in humans,[3] although the third
gene, SAA3, is believed to represent a
pseudogene that does not generate
messenger RNA or
protein.[4] Molecular weights of the human proteins are estimated at 11.7 kDa for SAA1[5] and 12.8 kDa for SAA4.[6]
Serum amyloid A (SAA) is also an acute phase marker that responds rapidly. Similar to
CRP, levels of acute-phase SAA increase within hours after inflammatory stimulus, and the magnitude of increase may be greater than that of CRP. Relatively trivial inflammatory stimuli can lead to SAA responses. It has been suggested that SAA levels correlate better with disease activity in early inflammatory joint disease than do
ESR and CRP. Although largely produced by hepatocytes, more recent studies show that SAA is produced by adipocytes as well, and its serum concentration is associated with
body mass index.[7]
Constitutive serum amyloid A proteins
A fourth SAA (SAA4) was identified in humans and is expressed constitutively in the liver and, thus, is defined as a constitutive SAA (C-SAA).[8] A similar protein that is now also called SAA4 has since been identified in the mouse; it had originally been designated SAA5.[9][10]
^Betts JC, Edbrooke MR, Thakker RV, Woo P (October 1991). "The human acute-phase serum amyloid A gene family: structure, evolution and expression in hepatoma cells". Scandinavian Journal of Immunology. 34 (4): 471–82.
doi:
10.1111/j.1365-3083.1991.tb01570.x.
PMID1656519.
S2CID26076389.
^Kluve-Beckerman B, Drumm ML, Benson MD (November 1991). "Nonexpression of the human serum amyloid A three (SAA3) gene". DNA and Cell Biology. 10 (9): 651–61.
doi:
10.1089/dna.1991.10.651.
PMID1755958.
^Pincus MR; McPherson RA; Henry JB (2007). Henry's Clinical Diagnosis and Management by Laboratory Methods. Saunders Elsevier.
ISBN978-1-4160-0287-1.
^Steel DM, Sellar GC, Uhlar CM, Simon S, DeBeer FC, Whitehead AS (May 1993). "A constitutively expressed serum amyloid A protein gene (SAA4) is closely linked to, and shares structural similarities with, an acute-phase serum amyloid A protein gene (SAA2)". Genomics. 16 (2): 447–54.
doi:
10.1006/geno.1993.1209.
PMID7686132.
^de Beer MC, de Beer FC, Gerardot CJ, Cecil DR, Webb NR, Goodson ML, Kindy MS (May 1996). "Structure of the mouse Saa4 gene and its linkage to the serum amyloid A gene family". Genomics. 34 (1): 139–42.
doi:
10.1006/geno.1996.0253.
PMID8661036.