SCHEMA is a computational
algorithm used in
protein engineering to identify fragments of
proteins (called schemas) that can be
recombined without disturbing the integrity of the proteins'
three-dimensional structure.[1] The algorithm calculates the
interactions between a protein's different
amino acid residues to determine which interactions may be disrupted by swapping structural
domains of the protein. By minimizing these disruptions, SCHEMA can be used to engineer
chimeric proteins that stably
fold and may have altered function relative to their parent proteins.[2] SCHEMA algorithm has been applied in the recombinant libraries of distantly related β-lactamases.[3]
References
^Voigt, CA; Martinez, C; Wang, ZG; Mayo, SL; Arnold, FH; et al. (June 2002). "Protein building blocks preserved by recombination". Nature Structural Biology. 9 (7): 553–558.
doi:
10.1038/nsb805.
PMID12042875.
S2CID19170079.