ROSA26 is a locus used for constitutive, ubiquitous gene expression in mice. [1] It was first isolated in 1991 [2] by the group of Philippe Soriano in a gene-trap mutagenesis screen of embryonic stem cells (ESCs). Over 800 knock-in lines have been created based on the ROSA26 locus according to the MGI database. [3] The human homolog of the ROSA26 locus has been identified. [4] ROSA stands for Reverse Orientation Splice Acceptor, named after the lentivirus genetrap vector. [5]
References
- ^ "rosa26". Retrieved 8 January 2013.
- ^ Friedrich, G; Soriano, P (1991). "Promoter traps in embryonic stem cells: A genetic screen to identify and mutate developmental genes in mice". Genes & Development. 5 (9): 1513–23. doi: 10.1101/gad.5.9.1513. PMID 1653172.
- ^ "Gt(ROSA)26Sor MGI Mouse Gene Detail - MGI:104735 - gene trap ROSA 26, Philippe Soriano". www.informatics.jax.org. Retrieved 2023-09-30.
- ^ Irion, Stefan; Luche, Hervé; Gadue, Paul; Fehling, Hans Joerg; Kennedy, Marion; Keller, Gordon (2007). "Identification and targeting of the ROSA26 locus in human embryonic stem cells". Nature Biotechnology. 25 (12): 1477–82. doi: 10.1038/nbt1362. PMID 18037879. S2CID 10292785.
- ^ Friedrich, G.; Soriano, P. (September 1991). "Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice". Genes & Development. 5 (9): 1513–1523. doi: 10.1101/gad.5.9.1513. ISSN 0890-9369. PMID 1653172.