From Wikipedia, the free encyclopedia
Protein-coding gene in the species Homo sapiens
POLI
Available structures
PDB Ortholog search:
PDBe
RCSB List of PDB id codes
1T3N ,
1ZET ,
2ALZ ,
2DPI ,
2DPJ ,
2FLL ,
2FLN ,
2FLP ,
2KHU ,
2KHW ,
2KTF ,
2L0G ,
2MBB ,
3EPG ,
3EPI ,
3G6X ,
3G6Y ,
3GV5 ,
3GV7 ,
3GV8 ,
3H40 ,
3H4B ,
3H4D ,
3NGD ,
3OSN ,
3Q8P ,
3Q8Q ,
3Q8R ,
3Q8S ,
4EBC ,
4EBD ,
4EBE ,
4EYH ,
4EYI ,
4FS1 ,
4FS2
Identifiers
Aliases
POLI , RAD30B, RAD3OB, polymerase (DNA) iota, DNA polymerase iotaExternal IDs
OMIM :
605252 ;
MGI :
1347081 ;
HomoloGene :
5209 ;
GeneCards :
POLI ;
OMA :
POLI - orthologs
Wikidata
DNA polymerase iota is an
enzyme that in humans is encoded by the POLI
gene .
[4] It is found in higher eukaryotes, and is believed to have arisen from a gene duplication from
Pol η . Pol ι, is a
Y family polymerase that is involved in
translesion synthesis . It can bypass 6-4 pyrimidine adducts and abasic sites and has a high frequency of wrong base incorporation. Like many other Y family polymerases Pol ι, has low processivity, a large DNA binding pocket and doesn't undergo conformational changes when DNA binds. These attributes are what allow Pol ι to carry out its task as a translesion polymerase. Pol ι only uses
Hoogsteen base pairing , during DNA synthesis, it will add
adenine opposite to
thymine in the syn conformation and can add both
cytosine and thymine in the anti conformation across
guanine , which it flips to the syn conformation.
Xeroderma pigmentosum variant
Xeroderma pigmentosum variant (XPV) cells lack
DNA polymerase eta (η).
[5] Instead these cells use DNA polymerase iota (ι).
[5] Exposure of XPV cells to UV light causes a very high frequency and unique spectrum of UV-induced mutations that can ultimately lead to malignant transformation.
[5]
References
^
a
b
c
GRCm38: Ensembl release 89: ENSMUSG00000038425 –
Ensembl , May 2017
^
"Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .
^
"Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .
^ Frank EG, Woodgate R (Aug 2007).
"Increased catalytic activity and altered fidelity of human DNA polymerase iota in the presence of manganese" . J Biol Chem . 282 (34): 24689–96.
doi :
10.1074/jbc.M702159200 .
PMID
17609217 .
^
a
b
c Wang Y, Woodgate R, McManus TP, Mead S, McCormick JJ, Maher VM. Evidence that in xeroderma pigmentosum variant cells, which lack DNA polymerase eta, DNA polymerase iota causes the very high frequency and unique spectrum of UV-induced mutations. Cancer Res. 2007 Apr 1;67(7):3018-26. doi: 10.1158/0008-5472.CAN-06-3073. PMID: 17409408
Further reading
McIntyre J (2020).
"Polymerase iota - an odd sibling among Y family polymerases" . DNA Repair . 86 (102753): 102753.
doi :
10.1016/j.dnarep.2019.102753 .
PMID
31805501 .
McDonald JP, Tissier A, Frank EG, et al. (2001).
"DNA polymerase iota and related rad30-like enzymes" . Philos. Trans. R. Soc. Lond. B Biol. Sci . 356 (1405): 53–60.
doi :
10.1098/rstb.2000.0748 .
PMC
1087691 .
PMID
11205331 .
Tissier A, Frank EG, McDonald JP, et al. (2001). "Biochemical characterization of human DNA polymerase iota provides clues to its biological function". Biochem. Soc. Trans . 29 (Pt 2): 183–7.
doi :
10.1042/BST0290183 .
PMID
11356150 .
McDonald JP, Rapić-Otrin V, Epstein JA, et al. (1999).
"Novel human and mouse homologs of Saccharomyces cerevisiae DNA polymerase eta" . Genomics . 60 (1): 20–30.
doi :
10.1006/geno.1999.5906 .
PMID
10458907 .
Masutani C, Kusumoto R, Iwai S, Hanaoka F (2000).
"Mechanisms of accurate translesion synthesis by human DNA polymerase eta" . EMBO J . 19 (12): 3100–9.
doi :
10.1093/emboj/19.12.3100 .
PMC
203367 .
PMID
10856253 .
Tissier A, McDonald JP, Frank EG, Woodgate R (2000).
"poliota, a remarkably error-prone human DNA polymerase" . Genes Dev . 14 (13): 1642–50.
doi :
10.1101/gad.14.13.1642 .
PMC
316739 .
PMID
10887158 .
Tissier A, Frank EG, McDonald JP, et al. (2000).
"Misinsertion and bypass of thymine-thymine dimers by human DNA polymerase iota" . EMBO J . 19 (19): 5259–66.
doi :
10.1093/emboj/19.19.5259 .
PMC
302107 .
PMID
11013228 .
Hartley JL, Temple GF, Brasch MA (2001).
"DNA cloning using in vitro site-specific recombination" . Genome Res . 10 (11): 1788–95.
doi :
10.1101/gr.143000 .
PMC
310948 .
PMID
11076863 .
Wiemann S, Weil B, Wellenreuther R, et al. (2001).
"Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs" . Genome Res . 11 (3): 422–35.
doi :
10.1101/gr.GR1547R .
PMC
311072 .
PMID
11230166 .
Bebenek K, Tissier A, Frank EG, et al. (2001).
"5'-Deoxyribose phosphate lyase activity of human DNA polymerase iota in vitro" . Science . 291 (5511): 2156–9.
Bibcode :
2001Sci...291.2156B .
doi :
10.1126/science.1058386 .
PMID
11251121 .
S2CID
83716303 .
Simpson JC, Wellenreuther R, Poustka A, et al. (2001).
"Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing" . EMBO Rep . 1 (3): 287–92.
doi :
10.1093/embo-reports/kvd058 .
PMC
1083732 .
PMID
11256614 .
Frank EG, Tissier A, McDonald JP, et al. (2001).
"Altered nucleotide misinsertion fidelity associated with poliota-dependent replication at the end of a DNA template" . EMBO J . 20 (11): 2914–22.
doi :
10.1093/emboj/20.11.2914 .
PMC
125476 .
PMID
11387224 .
Vaisman A, Tissier A, Frank EG, et al. (2001).
"Human DNA polymerase iota promiscuous mismatch extension" . J. Biol. Chem . 276 (33): 30615–22.
doi :
10.1074/jbc.M102694200 .
PMID
11402031 .
Ohmori H, Friedberg EC, Fuchs RP, et al. (2001).
"The Y-family of DNA polymerases" . Mol. Cell . 8 (1): 7–8.
doi :
10.1016/S1097-2765(01)00278-7 .
PMID
11515498 .
Vaisman A, Woodgate R (2002).
"Unique misinsertion specificity of poliota may decrease the mutagenic potential of deaminated cytosines" . EMBO J . 20 (22): 6520–9.
doi :
10.1093/emboj/20.22.6520 .
PMC
125734 .
PMID
11707422 .
Faili A, Aoufouchi S, Flatter E, et al. (2002). "Induction of somatic hypermutation in immunoglobulin genes is dependent on DNA polymerase iota". Nature . 419 (6910): 944–7.
Bibcode :
2002Natur.419..944F .
doi :
10.1038/nature01117 .
PMID
12410315 .
S2CID
4431410 .
Kannouche P, Fernández de Henestrosa AR, Coull B, et al. (2003).
"Localization of DNA polymerases eta and iota to the replication machinery is tightly co-ordinated in human cells" . EMBO J . 21 (22): 6246–56.
doi :
10.1093/emboj/cdf618 .
PMC
137208 .
PMID
12426396 .
Frank EG, Sayer JM, Kroth H, et al. (2002).
"Translesion replication of benzo[a]pyrene and benzo[c]phenanthrene diol epoxide adducts of deoxyadenosine and deoxyguanosine by human DNA polymerase iota" . Nucleic Acids Res . 30 (23): 5284–92.
doi :
10.1093/nar/gkf643 .
PMC
137958 .
PMID
12466554 .
Strausberg RL, Feingold EA, Grouse LH, et al. (2003).
"Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences" . Proc. Natl. Acad. Sci. U.S.A . 99 (26): 16899–903.
Bibcode :
2002PNAS...9916899M .
doi :
10.1073/pnas.242603899 .
PMC
139241 .
PMID
12477932 .
PDB gallery
1t3n : Structure of the catalytic core of DNA polymerase Iota in complex with DNA and dTTP
1zet : X-ray data do not support hoogsteen base-pairing during replication by human polymerase iota
2alz : Ternary Complex of hPoli with DNA and dCTP
2dpi : Ternary complex of hPoli with DNA and dCTP
2dpj : structure of hPoli with DNA and dTTP
2fll : Ternary complex of human DNA polymerase iota with DNA and dTTP
2fln : binary complex of catalytic core of human DNA polymerase iota with DNA (template A)
2flp : Binary complex of the catalytic core of human DNA polymerase iota with DNA (template G)