In cellular metabolism, NAD is involved in redox reactions, carrying
electrons from one reaction to another, so it is found in two forms: NAD+ is an
oxidizing agent, accepting electrons from other molecules and becoming reduced; with H+, this reaction forms NADH, which can be used as a
reducing agent to donate electrons. These
electron transfer reactions are the main function of NAD. It is also used in other cellular processes, most notably as a
substrate of
enzymes in adding or removing
chemical groups to or from
proteins, in
posttranslational modifications. Because of the importance of these functions, the enzymes involved in NAD metabolism are targets for
drug discovery.
In organisms, NAD can be synthesized from simple building-blocks (
de novo) from either
tryptophan or
aspartic acid, each a case of an
amino acid. Alternatively, more complex components of the coenzymes are taken up from nutritive compounds such as
niacin; similar compounds are produced by reactions that break down the structure of NAD, providing a
salvage pathway that recycles them back into their respective active form.
Some NAD is converted into the coenzyme
nicotinamide adenine dinucleotide phosphate (NADP), whose chemistry largely parallels that of NAD, though its predominant role is as a coenzyme in
anabolic metabolism.
In the name NAD+, the
superscripted plus sign indicates the positive
formal charge on one of its nitrogen atoms.
The compound accepts or donates the equivalent of H−.[6] Such reactions (summarized in formula below) involve the removal of two hydrogen atoms from the reactant (R), in the form of a
hydride ion (H−), and a
proton (H+). The proton is released into solution, while the reductant RH2 is oxidized and NAD+ reduced to NADH by transfer of the hydride to the nicotinamide ring.
RH2 + NAD+ → NADH + H+ + R;
From the hydride electron pair, one electron is attracted to the slightly more electronegative atom of the nicotinamide ring of NAD+, becoming part of the nicotinamide moiety. The second electron and proton atom are transferred to the carbon atom adjacent to the N atom. The
midpoint potential of the NAD+/NADH redox pair is −0.32
volts, which makes NADH a moderately strong reducing agent.[7] The reaction is easily reversible, when NADH reduces another molecule and is re-oxidized to NAD+. This means the coenzyme can continuously cycle between the NAD+ and NADH forms without being consumed.[5]
In appearance, all forms of this coenzyme are white
amorphous powders that are
hygroscopic and highly water-soluble.[8] The solids are stable if stored dry and in the dark. Solutions of NAD+ are colorless and stable for about a week at 4
°C and neutral
pH, but decompose rapidly in acidic or alkaline solutions. Upon decomposition, they form products that are
enzyme inhibitors.[9]
Both NAD+ and NADH strongly absorb
ultraviolet light because of the adenine. For example, peak absorption of NAD+ is at a
wavelength of 259
nanometers (nm), with an
extinction coefficient of 16,900
M−1cm−1. NADH also absorbs at higher wavelengths, with a second peak in UV absorption at 339 nm with an extinction coefficient of 6,220 M−1cm−1.[10] This difference in the ultraviolet
absorption spectra between the oxidized and reduced forms of the coenzymes at higher wavelengths makes it simple to measure the conversion of one to another in
enzyme assays – by measuring the amount of UV absorption at 340 nm using a
spectrophotometer.[10]
NAD+ and NADH also differ in their
fluorescence. Freely diffusing NADH in aqueous solution, when excited at the nicotinamide absorbance of ~335 nm (near-UV), fluoresces at 445–460 nm (violet to blue) with a
fluorescence lifetime of 0.4
nanoseconds, while NAD+ does not fluoresce.[11][12] The properties of the fluorescence signal changes when NADH binds to
proteins, so these changes can be used to measure
dissociation constants, which are useful in the study of
enzyme kinetics.[12][13] These changes in fluorescence are also used to measure changes in the redox state of living cells, through
fluorescence microscopy.[14]
NADH can be converted to NAD+ in a reaction catalysed by copper, which requires hydrogen peroxide. Thus, the supply of NAD+ in cells requires mitochondrial copper(II).[15][16]
Concentration and state in cells
In rat liver, the total amount of NAD+ and NADH is approximately 1
μmole per
gram of wet weight, about 10 times the concentration of NADP+ and NADPH in the same cells.[17] The actual concentration of NAD+ in cell
cytosol is harder to measure, with recent estimates in animal cells ranging around 0.3
mM,[18][19] and approximately 1.0 to 2.0 mM in
yeast.[20] However, more than 80% of NADH fluorescence in mitochondria is from bound form, so the concentration in solution is much lower.[21]
NAD+ concentrations are highest in the mitochondria, constituting 40% to 70% of the total cellular NAD+.[22] NAD+ in the cytosol is carried into the mitochondrion by a specific
membrane transport protein, since the coenzyme cannot
diffuse across membranes.[23] The intracellular
half-life of NAD+ was claimed to be between 1–2 hours by one review,[24] whereas another review gave varying estimates based on compartment: intracellular 1–4 hours, cytoplasmic 2 hours, and mitochondrial 4–6 hours.[25]
The balance between the oxidized and reduced forms of nicotinamide adenine dinucleotide is called the NAD+/NADH ratio. This ratio is an important component of what is called the redox state of a cell, a measurement that reflects both the metabolic activities and the health of cells.[26] The effects of the NAD+/NADH ratio are complex, controlling the activity of several key enzymes, including
glyceraldehyde 3-phosphate dehydrogenase and
pyruvate dehydrogenase. In healthy mammalian tissues, estimates of the ratio of free NAD+ to NADH in the cytoplasm typically lie around 700:1; the ratio is thus favorable for oxidative reactions.[27][28] The ratio of total NAD+/NADH is much lower, with estimates ranging from 3–10 in mammals.[29] In contrast, the
NADP+/NADPH ratio is normally about 0.005, so NADPH is the dominant form of this coenzyme.[30] These different ratios are key to the different metabolic roles of NADH and NADPH.
Biosynthesis
NAD+ is synthesized through two metabolic pathways. It is produced either in a
de novo pathway from amino acids or in salvage pathways by recycling preformed components such as
nicotinamide back to NAD+. Although most tissues synthesize NAD+ by the salvage pathway in mammals, much more de novo synthesis occurs in the liver from tryptophan, and in the kidney and
macrophages from
nicotinic acid.[31]
De novo production
Most organisms synthesize NAD+ from simple components.[6] The specific set of reactions differs among organisms, but a common feature is the generation of
quinolinic acid (QA) from an amino acid – either
tryptophan (Trp) in animals and some bacteria, or
aspartic acid (Asp) in some bacteria and plants.[32][33] The quinolinic acid is converted to nicotinic acid mononucleotide (NaMN) by transfer of a phosphoribose moiety. An adenylate moiety is then transferred to form nicotinic acid adenine dinucleotide (NaAD). Finally, the nicotinic acid moiety in NaAD is
amidated to a nicotinamide (Nam) moiety, forming nicotinamide adenine dinucleotide.[6]
Despite the presence of the de novo pathway, the salvage reactions are essential in humans; a lack of
niacin in the diet causes the
vitamin deficiency disease
pellagra.[37] This high requirement for NAD+ results from the constant consumption of the coenzyme in reactions such as posttranslational modifications, since the cycling of NAD+ between oxidized and reduced forms in redox reactions does not change the overall levels of the coenzyme.[6]
The major source of NAD+ in mammals is the salvage pathway which recycles the
nicotinamide produced by enzymes utilizing NAD+.[38] The first step, and the rate-limiting enzyme in the salvage pathway is
nicotinamide phosphoribosyltransferase (NAMPT), which produces
nicotinamide mononucleotide (NMN).[38] NMN is the immediate precursor to NAD+ in the salvage pathway.[39]
Besides assembling NAD+de novo from simple amino acid precursors, cells also salvage preformed compounds containing a pyridine base. The three vitamin precursors used in these salvage metabolic pathways are nicotinic acid (NA), nicotinamide (Nam) and
nicotinamide riboside (NR).[6] These compounds can be taken up from the diet and are termed vitamin B3 or niacin. However, these compounds are also produced within cells and by digestion of cellular NAD+. Some of the enzymes involved in these salvage pathways appear to be concentrated in the
cell nucleus, which may compensate for the high level of reactions that consume NAD+ in this
organelle.[40] There are some reports that mammalian cells can take up extracellular NAD+ from their surroundings,[41] and both nicotinamide and nicotinamide riboside can be absorbed from the gut.[42]
The salvage pathways used in
microorganisms differ from those of
mammals.[43] Some pathogens, such as the yeast Candida glabrata and the bacterium Haemophilus influenzae are NAD+auxotrophs – they cannot synthesize NAD+ – but possess salvage pathways and thus are dependent on external sources of NAD+ or its precursors.[44][45] Even more surprising is the intracellular
pathogenChlamydia trachomatis, which lacks recognizable candidates for any genes involved in the biosynthesis or salvage of both NAD+ and NADP+, and must acquire these coenzymes from its
host.[46]
Functions
Nicotinamide adenine dinucleotide has several essential roles in
metabolism. It acts as a
coenzyme in
redox reactions, as a donor of ADP-ribose moieties in
ADP-ribosylation reactions, as a precursor of the
second messenger molecule
cyclic ADP-ribose, as well as acting as a substrate for bacterial
DNA ligases and a group of enzymes called
sirtuins that use NAD+ to remove
acetyl groups from proteins. In addition to these metabolic functions, NAD+ emerges as an adenine nucleotide that can be released from cells spontaneously and by regulated mechanisms,[48][49] and can therefore have important
extracellular roles.[49]
The main role of NAD+ in metabolism is the transfer of electrons from one molecule to another. Reactions of this type are catalyzed by a large group of enzymes called
oxidoreductases. The correct names for these enzymes contain the names of both their substrates: for example
NADH-ubiquinone oxidoreductase catalyzes the oxidation of NADH by
coenzyme Q.[50] However, these enzymes are also referred to as dehydrogenases or reductases, with NADH-ubiquinone oxidoreductase commonly being called NADH dehydrogenase or sometimes coenzyme Q reductase.[51]
There are many different superfamilies of enzymes that bind NAD+ / NADH. One of the most common superfamilies includes a
structural motif known as the
Rossmann fold.[52][53] The motif is named after
Michael Rossmann, who was the first scientist to notice how common this structure is within nucleotide-binding proteins.[54]
When bound in the active site of an oxidoreductase, the nicotinamide ring of the coenzyme is positioned so that it can accept a hydride from the other substrate. Depending on the enzyme, the hydride donor is positioned either "above" or "below" the plane of the planar C4 carbon, as defined in the figure. Class A oxidoreductases transfer the atom from above; class B enzymes transfer it from below. Since the C4 carbon that accepts the hydrogen is
prochiral, this can be exploited in
enzyme kinetics to give information about the enzyme's mechanism. This is done by mixing an enzyme with a substrate that has
deuterium atoms substituted for the hydrogens, so the enzyme will reduce NAD+ by transferring deuterium rather than hydrogen. In this case, an enzyme can produce one of two
stereoisomers of NADH.[56]
Despite the similarity in how proteins bind the two coenzymes, enzymes almost always show a high level of specificity for either NAD+ or NADP+.[57] This specificity reflects the distinct metabolic roles of the respective coenzymes, and is the result of distinct sets of
amino acid residues in the two types of coenzyme-binding pocket. For instance, in the active site of NADP-dependent enzymes, an
ionic bond is formed between a basic amino acid side-chain and the acidic phosphate group of NADP+. On the converse, in NAD-dependent enzymes the charge in this pocket is reversed, preventing NADP+ from binding. However, there are a few exceptions to this general rule, and enzymes such as
aldose reductase,
glucose-6-phosphate dehydrogenase, and
methylenetetrahydrofolate reductase can use both coenzymes in some species.[58]
The redox reactions catalyzed by oxidoreductases are vital in all parts of metabolism, but one particularly important area where these reactions occur is in the release of energy from nutrients. Here, reduced compounds such as
glucose and
fatty acids are oxidized, thereby releasing energy. This energy is transferred to NAD+ by reduction to NADH, as part of
beta oxidation,
glycolysis, and the
citric acid cycle. In
eukaryotes the electrons carried by the NADH that is produced in the
cytoplasm are transferred into the
mitochondrion (to reduce mitochondrial NAD+) by
mitochondrial shuttles, such as the
malate-aspartate shuttle.[59] The mitochondrial NADH is then oxidized in turn by the
electron transport chain, which pumps protons across a membrane and generates ATP through
oxidative phosphorylation.[60] These shuttle systems also have the same transport function in
chloroplasts.[61]
Since both the oxidized and reduced forms of nicotinamide adenine dinucleotide are used in these linked sets of reactions, the cell maintains significant concentrations of both NAD+ and NADH, with the high NAD+/NADH ratio allowing this coenzyme to act as both an oxidizing and a reducing agent.[62] In contrast, the main function of NADPH is as a reducing agent in
anabolism, with this coenzyme being involved in pathways such as
fatty acid synthesis and
photosynthesis. Since NADPH is needed to drive redox reactions as a strong reducing agent, the NADP+/NADPH ratio is kept very low.[62]
Although it is important in catabolism, NADH is also used in anabolic reactions, such as
gluconeogenesis.[63] This need for NADH in anabolism poses a problem for prokaryotes growing on nutrients that release only a small amount of energy. For example,
nitrifying bacteria such as Nitrobacter oxidize nitrite to nitrate, which releases sufficient energy to pump protons and generate ATP, but not enough to produce NADH directly.[64] As NADH is still needed for anabolic reactions, these bacteria use a
nitrite oxidoreductase to produce enough
proton-motive force to run part of the electron transport chain in reverse, generating NADH.[65]
Non-redox roles
The coenzyme NAD+ is also consumed in ADP-ribose transfer reactions. For example, enzymes called
ADP-ribosyltransferases add the ADP-ribose moiety of this molecule to proteins, in a
posttranslational modification called
ADP-ribosylation.[66] ADP-ribosylation involves either the addition of a single ADP-ribose moiety, in mono-ADP-ribosylation, or the transferral of ADP-ribose to proteins in long branched chains, which is called poly(ADP-ribosyl)ation.[67] Mono-ADP-ribosylation was first identified as the mechanism of a group of bacterial
toxins, notably
cholera toxin, but it is also involved in normal
cell signaling.[68][69] Poly(ADP-ribosyl)ation is carried out by the
poly(ADP-ribose) polymerases.[67][70] The poly(ADP-ribose) structure is involved in the regulation of several cellular events and is most important in the
cell nucleus, in processes such as
DNA repair and
telomere maintenance.[70] In addition to these functions within the cell, a group of
extracellular ADP-ribosyltransferases has recently been discovered, but their functions remain obscure.[71]
NAD+ may also be added onto cellular
RNA as a 5'-terminal modification.[72]
NAD+ is also consumed by different NAD+-consuming enzymes, such as
CD38,
CD157,
PARPs and the NAD-dependent
deacetylases (
sirtuins,such as
Sir2.[76]).[77] These enzymes act by transferring an
acetyl group from their substrate protein to the ADP-ribose moiety of NAD+; this cleaves the coenzyme and releases nicotinamide and O-acetyl-ADP-ribose. The sirtuins mainly seem to be involved in regulating
transcription through deacetylating histones and altering
nucleosome structure.[78] However, non-histone proteins can be deacetylated by sirtuins as well. These activities of sirtuins are particularly interesting because of their importance in the regulation of
aging.[79][80]
Other NAD-dependent enzymes include bacterial
DNA ligases, which join two DNA ends by using NAD+ as a substrate to donate an
adenosine monophosphate (AMP) moiety to the 5' phosphate of one DNA end. This intermediate is then attacked by the 3' hydroxyl group of the other DNA end, forming a new
phosphodiester bond.[81] This contrasts with
eukaryotic DNA ligases, which use ATP to form the DNA-AMP intermediate.[82]
Li et al. have found that NAD+ directly regulates protein-protein interactions.[83] They also show that one of the causes of age-related decline in DNA repair may be increased binding of the protein
DBC1 (Deleted in Breast Cancer 1) to
PARP1 (poly[ADP–ribose] polymerase 1) as NAD+ levels decline during aging.[83] The decline in cellular concentrations of NAD+ during aging likely contributes to the
aging process and to the
pathogenesis of the chronic diseases of aging.[84] Thus, the modulation of NAD+ may protect against cancer, radiation, and aging.[83]
Extracellular actions of NAD+
In recent years, NAD+ has also been recognized as an
extracellular signaling molecule involved in cell-to-cell communication.[49][85][86] NAD+ is released from
neurons in
blood vessels,[48]urinary bladder,[48][87]large intestine,[88][89] from neurosecretory cells,[90] and from brain
synaptosomes,[91] and is proposed to be a novel
neurotransmitter that transmits information from
nerves to effector cells in
smooth muscle organs.[88][89] In plants, the extracellular nicotinamide adenine dinucleotide induces resistance to pathogen infection and the first extracellular NAD receptor has been identified.[92] Further studies are needed to determine the underlying mechanisms of its extracellular actions and their importance for human health and life processes in other organisms.
Clinical significance
The enzymes that make and use NAD+ and NADH are important in both
pharmacology and the research into future treatments for disease.[93]Drug design and drug development exploits NAD+ in three ways: as a direct target of drugs, by designing
enzyme inhibitors or activators based on its structure that change the activity of NAD-dependent enzymes, and by trying to inhibit NAD+ biosynthesis.[94]
Because cancer cells utilize increased
glycolysis, and because NAD enhances glycolysis, nicotinamide phosphoribosyltransferase (NAD salvage pathway) is often amplified in cancer cells.[95][96]
Since many oxidoreductases use NAD+ and NADH as substrates, and bind them using a highly conserved structural motif, the idea that inhibitors based on NAD+ could be specific to one enzyme is surprising.[102] However, this can be possible: for example, inhibitors based on the compounds
mycophenolic acid and
tiazofurin inhibit
IMP dehydrogenase at the NAD+ binding site. Because of the importance of this enzyme in
purine metabolism, these compounds may be useful as anti-cancer, anti-viral, or
immunosuppressive drugs.[102][103] Other drugs are not enzyme inhibitors, but instead activate enzymes involved in NAD+ metabolism.
Sirtuins are a particularly interesting target for such drugs, since activation of these NAD-dependent deacetylases extends lifespan in some animal models.[104] Compounds such as
resveratrol increase the activity of these enzymes, which may be important in their ability to delay aging in both vertebrate,[105] and invertebrate
model organisms.[106][107] In one experiment, mice given NAD for one week had improved nuclear-mitochrondrial communication.[108]
Because of the differences in the
metabolic pathways of NAD+ biosynthesis between organisms, such as between bacteria and humans, this area of metabolism is a promising area for the development of new
antibiotics.[109][110] For example, the enzyme
nicotinamidase, which converts nicotinamide to nicotinic acid, is a target for drug design, as this enzyme is absent in humans but present in yeast and bacteria.[43]
In bacteriology, NAD, sometimes referred to factor V, is used as a supplement to culture media for some
fastidious bacteria.[111]
The coenzyme NAD+ was first discovered by the British biochemists
Arthur Harden and
William John Young in 1906.[112] They noticed that adding boiled and filtered
yeast extract greatly accelerated
alcoholic fermentation in unboiled yeast extracts. They called the unidentified factor responsible for this effect a coferment. Through a long and difficult purification from yeast extracts, this heat-stable factor was identified as a
nucleotide sugar phosphate by
Hans von Euler-Chelpin.[113] In 1936, the German scientist
Otto Heinrich Warburg showed the function of the nucleotide coenzyme in hydride transfer and identified the nicotinamide portion as the site of redox reactions.[114]
Vitamin precursors of NAD+ were first identified in 1938, when
Conrad Elvehjem showed that liver has an "anti-black tongue" activity in the form of nicotinamide.[115] Then, in 1939, he provided the first strong evidence that niacin is used to synthesize NAD+.[116] In the early 1940s,
Arthur Kornberg was the first to detect an enzyme in the biosynthetic pathway.[117] In 1949, the American biochemists Morris Friedkin and
Albert L. Lehninger proved that NADH linked metabolic pathways such as the
citric acid cycle with the synthesis of ATP in oxidative phosphorylation.[118] In 1958, Jack Preiss and Philip Handler discovered the intermediates and enzymes involved in the biosynthesis of NAD+;[119][120] salvage synthesis from nicotinic acid is termed the Preiss-Handler pathway. In 2004,
Charles Brenner and co-workers uncovered the
nicotinamide riboside kinase pathway to NAD+.[121]
The non-redox roles of NAD(P) were discovered later.[5] The first to be identified was the use of NAD+ as the ADP-ribose donor in ADP-ribosylation reactions, observed in the early 1960s.[122] Studies in the 1980s and 1990s revealed the activities of NAD+ and NADP+ metabolites in cell signaling – such as the action of
cyclic ADP-ribose, which was discovered in 1987.[123]
The metabolism of NAD+ remained an area of intense research into the 21st century, with interest heightened after the discovery of the NAD+-dependent protein deacetylases called
sirtuins in 2000, by Shin-ichiro Imai and coworkers in the laboratory of
Leonard P. Guarente.[124] In 2009 Imai proposed the "NAD World" hypothesis that key regulators of aging and longevity in mammals are
sirtuin 1 and the primary NAD+ synthesizing enzyme
nicotinamide phosphoribosyltransferase (NAMPT).[125] In 2016 Imai expanded his hypothesis to "NAD World 2.0", which postulates that extracellular NAMPT from
adipose tissue maintains NAD+ in the
hypothalamus (the control center) in conjunction with
myokines from
skeletal muscle cells.[126] In 2018, Napa Therapeutics was formed to develop drugs against a novel aging related target based on the research in NAD metabolism conducted in the lab of
Eric Verdin.[127]
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