The following table lists all of the cities in the Midwestern United States with at least 100,000 people. These numbers were taken directly from the United States Census Bureau. ^[1] Note that only people living in the city itself are counted. People living in suburbs are not included.

Rank	City	State	Census Population		Change
			2010	2020
1	Chicago	Illinois	2,695,598	2,746,388	+1.9%
2	Columbus	Ohio	787,033	905,748	+15.1%
3	Indianapolis	Indiana	820,445	887,642	+8.2%
4	Detroit	Michigan	713,777	639,111	−10.5%
5	Milwaukee	Wisconsin	594,833	577,222	−3.0%
6	Kansas City	Missouri	459,787	508,090	+10.5%
7	Omaha	Nebraska	408,958	486,051	+18.9%
8	Minneapolis	Minnesota	382,578	429,954	+12.4%
9	Wichita	Kansas	382,368	397,532	+4.0%
10	Cleveland	Ohio	396,815	372,624	−6.1%
11	Saint Paul	Minnesota	285,068	311,527	+9.3%
12	Cincinnati	Ohio	296,943	309,317	+4.2%
13	St. Louis	Missouri	319,294	301,578	−5.5%
14	Lincoln	Nebraska	258,379	291,082	+12.7%
15	Toledo	Ohio	287,208	270,871	−5.7%
16	Madison	Wisconsin	233,209	269,840	+15.7%
17	Fort Wayne	Indiana	253,691	263,886	+4.0%
18	Des Moines	Iowa	203,433	214,133	+5.3%
19	Grand Rapids	Michigan	188,040	198,917	+5.8%
20	Overland Park	Kansas	173,372	197,238	+13.8%
21	Sioux Falls	South Dakota	153,888	192,517	+25.1%
22	Akron	Ohio	199,110	190,469	−4.3%
23	Aurora	Illinois	197,899	180,542	−8.8%
24	Springfield	Missouri	159,498	169,176	+6.1%
25	Kansas City	Kansas	145,786	156,607	+7.4%
26	Joliet	Illinois	147,433	150,362	+2.0%
27	Naperville	Illinois	141,853	149,540	+5.4%
28	Rockford	Illinois	152,871	148,655	−2.8%
29	Olathe	Kansas	125,872	141,290	+12.2%
30	Warren	Michigan	134,056	139,387	+4.0%
31	Cedar Rapids	Iowa	126,326	137,710	+9.0%
32	Dayton	Ohio	141,527	137,644	−2.7%
33	Sterling Heights	Michigan	129,699	134,346	+3.6%
34	Topeka	Kansas	127,473	126,587	−0.7%
35	Columbia	Missouri	108,500	126,254	+16.4%
36	Fargo	North Dakota	105,549	125,990	+19.4%
37	Ann Arbor	Michigan	113,934	123,851	+8.7%
38	Independence	Missouri	116,830	123,011	+5.3%
39	Rochester	Minnesota	106,769	121,395	+13.7%
40	Evansville	Indiana	117,429	117,298	−0.1%
41	Elgin	Illinois	108,188	114,797	+6.1%
42	Springfield	Illinois	116,250	114,394	−1.6%
43	Peoria	Illinois	115,007	113,150	−1.6%
44	Lansing	Michigan	114,297	112,644	−1.4%
45	Dearborn	Michigan	98,153	109,976	+12.0%
46	Green Bay	Wisconsin	104,057	107,395	+3.2%
47	South Bend	Indiana	101,168	103,453	+2.3%
48	Davenport	Iowa	99,685	101,724	+2.0%
49	Lee's Summit	Missouri	91,364	101,108	+10.7%
50	Clinton Township	Michigan	96,796	100,513	+3.8%

References