Tobin studies how
phytochrome photoreceptors interact with the
circadian clock in plants, in particular circadian oscillator proteins and the ways in which feedback loops are regulated through
gene expression.[4] Tobin identified one of the first two components of the circadian clock in plants, the dawn expressed
transcription factorCCA1.[6][7] Her lab also showed that CCA1 was necessary for phytochrome response in Arabidopsis thaliana[8] and that one type of regulation involves the phosphorylation of CCA1 by the protein kinase
CK2.[7][9]
Early life and education
Elaine Munsey was born in
Louisville, Kentucky on December 23, 1944.[3] Her family had immigrated from
Odessa and
Lithuania.[1] Munsey's interests included science, mathematics and basketball. She attended the
1960 Democratic National Convention as a volunteer working for
Adlai Stevenson II's presidential campaign. While in high school, she also participated in civil rights marches and heard
Martin Luther King Jr. speak in Louisville.[1]
She graduated from Seneca High School in Louisville in 1962.[10]
She was accepted into the Biology Department at Stanford. She took classes in plant physiology with
Winslow Briggs, worked in his laboratory, and transferred to Harvard when Briggs took a professorship there. In 1968 she married Allan J. Tobin. They spent a year at the
Weizmann Institute of Science in Israel, where Elaine Tobin worked with plant geneticist Ezra Galun.[1] After returning to North America, she completed her Ph.D. in Biology at
Harvard University in 1972.[3] She later married J. Philip Thornber.[2]
Career
In 1973 Tobin went to
Brandeis University, where she did postdoctoral work with Attila Klein, on the influence of light on the development of plants.
In 1975 she was hired in the Biology Department at
University of California, Los Angeles (UCLA). Support was sparse, but she was able to get funding for basic research on plants from the
National Institutes of Health (NIH). She was able to obtain laboratory space previously used by retiring professor Karl Hamner.[11]
As a student with Winslow Briggs, Tobin had been introduced to the effects of phytochrome on flowering and to the work of Karl Hamner on circadian rhythms and flowering.[1]
Circadian rhythms in plants help them to coordinate with external light/dark cycles. Anticipating dawn, dusk, and seasonal day length allows plants to more effectively regulate both daily and seasonal activities, including the movement of leaves and petals, the opening of
stomata for
photosynthesis,
stem growth, and the
development of flowers.[12]
Tobin first used Lemna gibba (duckweed) and later Arabidopsis thaliana (cress) as
model plant systems to study light regulation of gene expression in plants, examining interactions between
phytochrome photoreceptors, genes, and circadian rhythms.[13]
Tobin was able to isolate poly(A) RNA from duckweed, expose
slab gels to x-ray film, and show that while some
mRNAs decreased in light, others increased.[13]
In 1984, postdoctoral student
Jane Silverthorne and Tobin demonstrated that photoreceptors in plants could affect the
transcription of specific genes. Light-harvesting chlorophyll a/b-binding (LHCB) protein sequences from Lemna gibba were low in darkness but could be rapidly and reversibly restored by light exposure.[13]
Tobin's group also demonstrated phytochrome regulation of LHCB proteins (also known as cab genes) in Arabidopsis.[13][14][15]
By growing duckweed heterotrophically in the dark, and exposing it briefly to red and
far-red light, Tobin demonstrated the effects of phytochromes on plant growth and transcription in rcbs genes.[16]
In a series of experiments beginning in 1993, Tobin's lab described DNA-binding activity with an affinity for LHCB in plant cells. Using a DNA fragment, they screened the Arabidopsis
expression library, and cloned a protein with relevant binding activity, which they named CCA1. They showed that
Circadian Clock Associated 1 (CCA1) was necessary for phytochrome response in Arabidopsis thaliana.[8]
Reports on the activity of CCA1 and a closely related gene (LHY) from
George Coupland were submitted together to Cell in 1998.[1] The two genes were the first two components of the circadian clock or central oscillator mechanism in plants to be identified.[6][7] Among many other studies of the regulation and function of CCA1, Tobin has determined that one method of clock regulation involves the phosphorylation of CCA1 by the protein kinase CK2.[17]
Tobin, E M; Silverthorne, J (1 June 1985). "Light Regulation of Gene Expression in Higher Plants". Annual Review of Plant Physiology. 36 (1): 569–593.
doi:
10.1146/annurev.pp.36.060185.003033.
ISSN0066-4294.
^
abThornber, J. Philip (2000). "Thirty Years of Fun with Antenna Pigment-Proteins and Photochemical Reaction Centers: A Tribute to the People Who Have Influenced My Career". Discoveries in Plant Biology. Vol. 3. World Scientific. pp. 325–346.
doi:
10.1142/9789812813503_0017.
ISBN978-981-02-3882-7.
^
abcdAmerican men & women of science: a biographical directory of today's leaders in physical, biological, and related sciences (24th ed.). Detroit, Mich.: Thomson Gale. 2007.
ISBN978-1-4144-3399-8.
^
abcdSage, Linda C. (2 December 2012).
"32. Gene regulation". Pigment of the Imagination: A History of Phytochrome Research. Elsevier. pp. 480–515.
ISBN978-0-323-13854-3. Retrieved 23 May 2022.