Scanning electron cryomicroscopy (CryoSEM) is a form of
electron microscopy where a
hydrated but cryogenically fixed sample is imaged on a
scanning electron microscope's cold stage in a
cryogenic chamber. The cooling is usually achieved with
liquid nitrogen.[1] CryoSEM of biological samples with a high moisture content can be done faster with fewer sample preparation steps than conventional SEM. In addition, the dehydration processes needed to prepare a biological sample for a conventional SEM chamber create numerous distortions in the tissue leading to structural artifacts during imaging.[2][3][4][5]
^Lesemann, Dietrich-Eckhardt; Mendgen, Kurt, eds. (1991). Electron Microscopy of Plant Pathogens. Berlin, Heidelberg: Springer Berlin Heidelberg.
ISBN978-3-642-75818-8.
^Schatten, Heide (2013). Scanning electron microscopy for the life sciences (Online-Ausg. ed.). Cambridge: Cambridge University Press.
ISBN9780521195997.
^Schatten, Heide; Pawley, James, eds. (2007). Biological low voltage field emission scanning electron microscopy. New York: Springer.
ISBN9780387729725.