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Greensboro, North Carolina, is a city located in Guilford County in the U.S. state of North Carolina. As of the 2000 census, the city had a total population of 223,891 making it the third most populous city in North Carolina. Its estimated population in 2004 was 231,543. It is located at the intersection of two interstate highways ( I-85 and I-40) in the Piedmont ("foot of the mountains") region, in Guilford County in the central part of the state. In 1808, Greensborough (as it was spelled then) replaced Guilford Court House as the county seat.